RNA 剪接因子 SRRM3 和 SRRM4 能够作为去势抵抗性的神经内分泌性前列腺癌的分子表征

RNA Splicing Factors SRRM3 and SRRM4 Distinguish Molecular Phenotypes of Castration-Resistant Neuroendocrine Prostate Cancer

原文链接：
online: Cancer Research

abbreviation(缩略表)：

• NE
  Neuroendocrine, 神经内分泌性
• mCRPC
  metastatic castration-resistant prostate cancer，转移性去势（去除雄性激素干扰，例如阉割的方法）抵抗性前列腺癌
• AR
  androgen receptor，雄性激素受体
• REST
  RE1-silencing transcription factor，神经发育相关基因的转录抑制因子
• SRRM4
  serine/arginine repetitive matrix protein，SRRM4与REST在神经发育过程中相互负向调控（Bushra Raj et al., 2011, Mol Cel）
• androgen receptor (AR)-positive NE-positive amphicrine prostate cancer (AMPC)
• AR-negative small cell or neuroendocrine prostate cancer (SCNPC)
• five mCRPC molecular phenotypes:
  • AR-high prostate cancer (ARPC);
  • AR-low prostate cancer (ARLPC);
  • amphicrine prostate cancer (AMPC) composed of tumor cells coexpressing AR and NE programs (ARþ/NEþ);
  • double-negative prostate cancer (DNPC) that lacks AR and NE gene expression (AR/NE);
  • SCNPC(AR/NEþ)

material and method

cell line

• C4-2B
• Du145
• 22Rv1
• VCaP
• NCI-H660

Note: 细胞系鉴定。All cell lines used in this project were validated through short tandem repeat analysis using ATCC reference genomes and were routinely tested for Mycoplasma using the MycoFluor Mycoplasma Detection Kit (Invitrogen).

Transient transfections and lentiviral transductions 顺转和稳转

• siRNA knockdown